例如:"lncRNA", "apoptosis", "WRKY"

Microarray expression profiling of tissues from mice with uniparental duplications of chromosomes 7 and 11 to identify imprinted genes.

Mamm. Genome. 2001 Oct;12(10):758-64. doi:10.1007/s00335-001-3027-5
J D Choi 1 , L A Underkoffler , J N Collins , S M Marchegiani , N A Terry , C V Beechey , R J Oakey
J D Choi 1 , L A Underkoffler , J N Collins , S M Marchegiani , N A Terry , C V Beechey , R J Oakey
+ et al

[No authors listed]

Author information
  • 1 Division of Human Genetics, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.

摘要


Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this approach is to combine the new technology with established genetic tools to associate genes with specific biological function. In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes (Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and strain-matched samples and attention to detail in tissue dissection technique.